LILLE, France - A first-half penalty earned Lille a shock 1-0 home victory over holders Real Madrid in the Champions League group phase on Wednesday, ending the 15-times European Cup winners' 14-game unbeaten run in the competition.

Jonathan David converted from the spot three minutes into first-half stoppage time to hand Real, who had not lost in their previous 36 matches in all competitions, their first defeat since January.

The result put Ligue 1 side Lille on three points from two games in the new format of Europe's premier club competition.

Real, who won their opening match against VfB Stuttgart, are also on three points.

"On the penalty, I try to focus, to keep calm to take a good shot but I'm confident when it comes to taking penalties," David said. "It has not sunk in yet but it's a memorable night."

Real keeper Andriy Lunin said his team had lacked their usual aggression.

"It's difficult to analyze right after. (We lacked) aggression, creativity, in the end, what we always have," he said.

"To fight a bit more. In the second half we showed more desire, but in the first half we were worse, that's obvious. We want to win because we know what colors we're playing in. But that's okay. Courage, keep working and prepare for the next game."

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Carlo Ancelotti's side started with Kylian Mbappe, who joined from Ligue 1 champions Paris St Germain in June, on the bench after the France captain picked up a muscle injury last week.

Real's Vinicius Jr. had the first chance with a low shot in the sixth minute as the visitors controlled the pace and the second came when Endrick's close-range attempt was blocked by Lucas Chevalier.

Lille went close to opening the scoring after 26 minutes when Andriy Lunin made a spectacular double save to deny David, first from the forward's header, then by tipping away the Canadian's follow-up shot.

Their relentless pressure paid off in stoppage time when Eduardo Camavinga handled Edon Zhegrova's free kick.

A VAR review ensued and David confidently converted the resulting penalty.

The hosts continued to apply pressure in the second half, refusing to sit back and wait as Real enjoyed possession, cutting off the supply to England midfielder Jude Bellingham.

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Ancelotti sent Mbappe and Luka Modric on to replace Endrick and Eder Militao and Lille survived a late Real onslaught to hang on for the victory.